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Copyright © Alfred B. Cunningham, John E. Lennox, and Rockford J. Ross, Eds. 2001-2008
Building and Using a Batch Biofilm Growth Reactor (Mason
||wide mouth polycarbonate sample jar with cap
||#6 rubber stoppers
||1x3 inch microscope slides (1mm thick)
||fine tooth modeling saw
||magnetic stir bar (a 1 inch teflon bar works
|360 ml (approx)
||1/10 Nutrient Broth, liquid LB broth, or any other
*The ports in the reactor lid can be cut with a laser
engraver, drill, or drill press with 1 1/16 inch paddle-type
- Microscope slides are inserted into the slots which
have been cut into each of the four # 6 rubber
- The slide rubber stopper assemblies are placed into the
1 1/16 inch holes in the lid of the polyethylene jar.
Orient the slides in the desired direction. Orientation
parallel to the wall of the jar gives a less turbulent flow
when the reactor is in operation.
- Sufficient liquid medium of the desired type is added
to the jar to cover the slides to the desired depth (about
- A magnetic stir bar is placed in the jar, and the top
of the jar is screwed into place.
- The entire apparatus is now sterilized by
- In operation the medium in the reactor is stirred by
placing the reactor on a magnetic stirrer. The degree of
agitation is controlled by the rotation speed of the
magnetic stir bar.
- The reactor may be inoculated through one of the rubber
- Gram staining.
- Harvesting biofilm associated cells from the glass
slides by scraping and sonication and their enumeration by
the drop plate method.
Figure 1. Batch Biofilm Growth Reactor
Educational Program Curricula and Teaching Resources
Supported in part by the Waksman Foundation for
Developed in collaboration with Dr. John Lennox, Penn
© 1999-2008 Center
for Biofilm Engineering, http://www.cbe.montana.edu